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ssDNA vs dsDNA

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Estimated time: 3 minutes
CISCE: Class 12

Key Points: ssDNA vs dsDNA

Feature Single-Stranded DNA (ssDNA) Double-Stranded DNA (dsDNA)
Structure Single linear strand; randomly coiled. Two strands intertwined in a double helix, bound by hydrogen bonds.
Occurrence Found in a few viruses (e.g., $\phi$X174). Found in most organisms.
Stability & Stiffness Less stiff and less stable. Comparatively stiffer and more stable.
Purine:Pyrimidine Ratio Highly variable. Constant (ratio is 1:1).
Chargaff's Rule Does not follow Chargaff's rule. Follows Chargaff's rule.
Key Discovery First observed by Robert Sinsheimer (1959). Double helix model proposed by James Watson and Francis Crick (1953).
Other Characteristics Can be formed from dsDNA through a process called melting. Can "melt" into ssDNA at high temperature, high pH, or low salt.
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