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Technique of Tissue Culture

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CISCE: Class 12

Key Points: Technique of tissue culture

Step Main Requirement Key Process Outcome
Aseptic conditions Sterile laboratory, laminar airflow, autoclave Sterilization of glassware, instruments, media and explants using heat, chemicals and UV Contamination-free culture environment
Preparation of culture medium Nutrient media (MS, White’s, Nitsch), agar, growth regulators Media prepared with salts, sucrose, vitamins, auxins/cytokinins, pH 5.8 and autoclaved Suitable medium for tissue growth
Inoculation and culture Sterilized explant, laminar airflow cabinet Surface sterilization of tissue and transfer onto medium under aseptic conditions Initiation of tissue growth
Callus formation and regeneration Controlled temperature, light and hormones Callus formation followed by root/shoot differentiation using auxin–cytokinin balance Development of complete plant
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