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Operon Concept - The Lac Operon

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Estimated time: 4 minutes
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Key Points: The Lac Operon

  • The lac operon is a gene regulatory system in E. coli that controls lactose metabolism by regulating transcription of structural genes. It includes the regulator gene (lacI), operator, promoter, and structural genes (z, y, a).
  • The lacI gene produces a repressor protein that binds to the operator in the absence of lactose, preventing RNA polymerase from initiating transcription.
  • In the absence of lactose, the operon remains “off” because the repressor blocks RNA polymerase, so structural genes are not expressed.
  • In the presence of lactose, a small amount enters the cell via permease and is converted into allolactose by β-galactosidase.
  • Allolactose acts as an inducer by binding to the repressor and changing its shape, inactivating it so it cannot bind the operator.
  • Once the repressor is inactivated, RNA polymerase binds to the promoter and transcribes the structural genes, producing enzymes for lactose metabolism.
  • This leads to the synthesis of β-galactosidase, permease, and transacetylase, enabling efficient utilisation of lactose.

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