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Mechanism of DNA Replication

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CBSE: Class 12
CISCE: Class 12

Key Points: Mechanism of DNA Replication

  • Initiation at Origin: DNA replication begins at a specific site called the origin of replication; prokaryotes have a single origin, while eukaryotes have multiple origins (replicons).
  • Unwinding of DNA: The DNA double helix is unwound and strands are separated by helicase, while topoisomerase (DNA gyrase) relieves supercoiling, forming replication forks.
  • Primer Formation: A short RNA primer is synthesized by the enzyme primase to provide a free 3′-OH end for DNA synthesis.
  • Elongation of New Strands: DNA polymerase adds nucleotides in the 5′ → 3′ direction using parental strands as templates; synthesis is continuous on the leading strand and discontinuous on the lagging strand forming Okazaki fragments.
  • Completion and Ligation: RNA primers are removed, gaps are filled with DNA, and Okazaki fragments are joined by DNA ligase to form complete daughter DNA molecules.
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