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Transcription Unit and the Gene

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Estimated time: 12 minutes
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

The Transcription Unit

Transcription Unit: A specific segment of DNA that is transcribed into RNA. It is defined by three structural regions: a Promoter, the Structural Gene, and a Terminator.

Components of a Transcription Unit:

Component Location (relative to coding strand) Structure / Nature Function
Promoter 5′ end (Upstream) DNA sequence; contains TATA box (eukaryotes) or Pribnow box (prokaryotes) Provides binding site for RNA polymerase; initiates transcription; defines template vs coding strand
Structural Gene Between promoter and terminator DNA sequence encoding RNA Carries the information to be transcribed into RNA​
Terminator 3′ end (Downstream) DNA sequence; often palindromic Signals the end of transcription; causes RNA polymerase to dissociate

Schematic structure of a transcription unit

CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Template vs. Coding Strand

Feature Template Strand Coding Strand
Other names Antisense strand, Non-coding strand, Minus (−) strand Sense strand, Non-template strand, Plus (+) strand
Polarity 3′ → 5′ 5′ → 3′
Role in Transcription Acts as the actual template for RNA synthesis Does NOT serve as a template; displaced during transcription
Sequence relationship to mRNA Complementary to mRNA Same sequence as mRNA (except T → U)
Used as reference? No Yes - all coordinates (upstream/downstream) are defined with respect to the coding strand​
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Monocistronic vs. Polycistronic

Feature Monocistronic Polycistronic
Number of cistrons per mRNA 1 Many (2 or more)
Organism type Eukaryotes Prokaryotes (bacteria)
Gene arrangement Split genes (exons + introns) Continuous (no introns usually)
mRNA processing Required (splicing, capping, tailing) Not required
Example Human β-globin gene Lac operon in E. coli
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Exons and Introns (Eukaryotic Split Genes)

In eukaryotes, the structural gene is interrupted - it is a split gene:

  • Exons (Expressed sequences) - Coding sequences that appear in the mature, processed mRNA​
  • Introns (Intervening sequences) - Non-coding sequences that are removed during RNA processing; do not appear in mature mRNA
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Key Points: Transcription Unit

Component Location Function
Promoter At the 5′ end of the structural gene Provides binding site for RNA polymerase and initiates transcription
Structural Gene Between promoter and terminator Contains genetic information to be transcribed
Template Strand DNA strand with 3′ → 5′ polarity Serves as template for RNA synthesis
Coding Strand DNA strand with 5′ → 3′ polarity Does not code directly; used as reference strand
Terminator At the 3′ end of the coding strand Signals the end of transcription
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