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Transcription Unit and the Gene

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Estimated time: 12 minutes
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

The Transcription Unit

Transcription Unit: A specific segment of DNA that is transcribed into RNA. It is defined by three structural regions: a Promoter, the Structural Gene, and a Terminator.

Components of a Transcription Unit:

Component Location (relative to coding strand) Structure / Nature Function
Promoter 5′ end (Upstream) DNA sequence: contains TATA box (eukaryotes) or Pribnow box (prokaryotes) Provides a binding site for RNA polymerase; initiates transcription; defines template vs coding strand
Structural Gene Between promoter and terminator DNA sequence encoding RNA Carries the information to be transcribed into RNA​
Terminator 3′ end (Downstream) DNA sequence; often palindromic Signals the end of transcription; causes RNA polymerase to dissociate

Schematic structure of a transcription unit

CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Template vs. Coding Strand

Feature Template Strand Coding Strand
Other names Antisense strand, Non-coding strand, Minus (−) strand Sense strand, Non-template strand, Plus (+) strand
Polarity 3′ → 5′ 5′ → 3′
Role in Transcription Acts as the actual template for RNA synthesis Does NOT serve as a template; displaced during transcription
Sequence relationship to mRNA Complementary to mRNA Same sequence as mRNA (except T → U)
Used as a reference? No Yes - all coordinates (upstream/downstream) are defined with respect to the coding strand​
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Monocistronic vs. Polycistronic

Feature Monocistronic Polycistronic
Number of cistrons per mRNA 1 Many (2 or more)
Organism type Eukaryotes Prokaryotes (bacteria)
Gene arrangement Split genes (exons + introns) Continuous (no introns usually)
mRNA processing Required (splicing, capping, tailing) Not required
Example Human β-globin gene Lac operon in E. coli
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Exons and Introns (Eukaryotic Split Genes)

In eukaryotes, the structural gene is interrupted - it is a split gene:

  • Exons (Expressed sequences) - Coding sequences that appear in the mature, processed mRNA​
  • Introns (Intervening sequences) - Non-coding sequences that are removed during RNA processing; do not appear in mature mRNA
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Key Points: Transcription Unit

Component Location Function
Promoter At the 5′ end of the structural gene Provides a binding site for RNA polymerase and initiates transcription
Structural Gene Between promoter and terminator Contains genetic information to be transcribed
Template Strand DNA strand with 3′ → 5′ polarity Serves as a template for RNA synthesis
Coding Strand DNA strand with 5′ → 3′ polarity Does not code directly; used as a reference strand
Terminator At the 3′ end of the coding strand Signals the end of transcription
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