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Monohybrid Cross

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Estimated time: 8 minutes
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Definition: Monohybrid Experiments

Mendel's first experiments were with the varieties of garden pea that differed in only one visible character. These are known as monohybrid experiments.

CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Introduction

monohybrid cross is a genetic cross in which only one pair of contrasting characters is studied, such as tallness versus dwarfness in pea plants.​ Mendel used this type of cross to understand how a single trait passes from parents to offspring and to establish the basic laws of inheritance.

CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Mendel’s Monohybrid Experiment

Mendel selected pure-breeding pea plants that differed in one visible character, such as tall and dwarf plant height.​ He crossed the pure tall plant with the pure dwarf plant and observed the traits in the first filial generation and then in the second filial generation after self-pollination.​

Experimental sequence

  1. Select a pure tall plant, genotype TT.​
  2. Select a pure dwarf plant, genotype tt.​
  3. Cross TT × tt to obtain the F1 generation.​
  4. All F1 offspring become tall with genotype Tt.​
  5. Self-pollinate F1 plants, Tt × Tt, to obtain F2.​
  6. In F2, both tall and dwarf plants appear.

Parental generation (P1):

 

First filial generation (F1):

 
Cross Result
TT × tt All offspring are tall
Genotype of offspring Tt
Observation Only dominant trait appears

Second filial generation (F2)

 

F2 ratios:

Ratio type Value
Phenotypic ratio 3 : 1
Genotypic ratio 1 : 2 : 1
CBSE: Class 12
Maharashtra State Board: Class 12
CISCE: Class 12

Key Points: Monohybrid Cross

Parameter Monohybrid Cross
Meaning Cross involving 1 pair of contrasting characters
Example TT × tt (Tall × Dwarf)
Phenotypic Ratio 3:1 (Tall: Dwarf)
Genotypic Ratio 1: 2: 1
Conclusion Tallness is dominant; dwarfness is recessive

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Genotypes and Phenotypes [00:14:43]
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