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Mechanism of Translation

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CISCE: Class 12

Key Points: Mechanism of Translation

Stage Key Events Enzymes / Factors Involved
Activation of Amino Acids Amino acids are activated by ATP and attached to specific tRNA molecules Aminoacyl-tRNA synthetase, ATP, Mg²⁺
Role of Ribosome Ribosome provides sites for mRNA binding and peptide synthesis; has A site and P site rRNA, ribosomal proteins
Initiation mRNA binds to small ribosomal subunit; initiator tRNA binds to start codon (AUG) at P site; large subunit joins Initiation factors, Mg²⁺, GTP
Elongation Aminoacyl-tRNA binds to A site; peptide bond forms; ribosome translocates along mRNA Peptidyl transferase, elongation factors, GTP
Termination Stop codon (UAA, UAG, UGA) is reached; polypeptide released; ribosome dissociates Release factors, GTP
Post-translational Modification Polypeptide undergoes folding and chemical modifications Deformylase, peptidases
Protein Translocation Proteins synthesized on free or bound ribosomes are transported to correct cellular locations ER membrane, Golgi apparatus
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