मराठी

Overview of Biotechnology

Topics

Reproduction in Lower and Higher Plants
Reproduction in Lower and Higher Animals
Inheritance and Variation
- Heredity
- Genes and Genetic
- Back Cross and Test Cross
- Deviations from Mendel’s Findings
- Chromosomal Theory of Inheritance
- Chromosomes - The Carriers of Heredity
- Linkage and Crossing Over
- Autosomal Inheritance
- Sex Determination
- Sex Linked Inheritance
- Human Genetic Disorders
- Exceptions to Mendel's Principles > Multiple alleles
- Linkage
- Types of Linkage > Complete Linkage
- Types of Linkage > Incomplete Linkage
- Crossing Over
- Colour blindness
- Haemophilia
- Sex Determination in Birds
- Down's syndrome (Mongolian Idiocy)
- Turner's Syndrome
- Klinefelter's Syndrome
- Overview of Inheritance and Variation
Molecular Basis of Inheritance
- Deoxyribonucleic Acid (DNA)
- Griffith’s Experiment
- Packaging of DNA Helix
- DNA Replication
- Protein Synthesis
- Regulation of Gene Expression
- The Lac Operon
- Genomics
- Human Genome Project
- DNA Fingerprinting
- Genetic Code
- Mechanism of Translation
- Overview of Molecular Basis of Inheritance
Origin and Evolution of Life
- Origin and Evolution of Universe and Earth
- Theories of Origin of Life
- Chemical Evolution of Life (Self-assembly Theory of the Origin of Life)
- Darwin’s Theory of Natural Selection (Darwinism)
- Mutation Theory
- Modern Synthetic Theory of Evolution
- Organic Evolution
- Hardy Weinberg’s Principle
- Adaptive Radiation
- Evidence of Evolution > Morphological Evidences
- Speciation
- Geological Time Scale
- Theories of Biological Evolution
- Overview of Origin and Evolution of Life
Plant Water Relation
- Plant Water Relation
- Properties of Water
- Water Available to Roots for Absorption
- Imbibition
- Diffusion
- Osmosis
- Osmotic Pressure
- Turgidity and Flaccidity (Plasmolysis)
- Passive Transport
- Water Potential (ψ)
- Concept of Transpiration
- Path of Water Across the Root
- Kinds of Transpiration
- Forces Contributing to Ascent of Sap
- Transport of Mineral Ions
- Structure of Stomatal Apparatus
- Significance of Transpiration
- Transportation of Food and Other Substances
- Overview of Plant Water Relation
Plant Growth and Mineral Nutrition
- Plant Growth
- Phases of Plant Growth
- Conditions Necessary for Plant Growth
- Plant Growth Rate
- Plant Growth Curve
- Differentiation, De-differentiation, Re- Differentiation
- Plant Development
- Plant Plasticity
- Plant Hormones
- Auxins
- Gibberellins
- Cytokinins
- Ethylene
- Abscisic Acid (ABA)
- Photoperiodism
- Vernalization (Yarovization)
- Plant Mineral Nutrition
- Nitrogen Cycle
- Overview of Plant Growth and Mineral Nutrition
Respiration and Circulation
- Cellular Respiration
- Organs of Respiratory Exchange
- Human Respiratory System
- Mechanism of respiration-Breathing
- Regulation of Breathing / Respiration
- Modified Respiratory Movements
- Disorders of Respiratory System
- Transportation in Living Organisms
- Circulation in Animals
- Types of Closed Circulation
- Blood Circulatory System
- Composition of Blood > Plasma (The Liquid Portion of Blood)
- Composition of Blood > Cellular Elements: Red Blood Cells (Erythrocytes)
- Composition of Blood > Cellular Elements: White Blood Cells (Leukocytes)
- Composition of Blood > Cellular Elements: Blood Platelets (Thrombocytes)
- Human Heart
- Working mechanism of human heart
- Blood Vessels
- Blood Pressure (B.P.)
- Electrocardiogram (ECG)
- Lymph and Lymphatic System
- Mechanism of respiration - Internal respiration
- Mechanism of respiration - External respiration
- Cellular Respiration
- Overview of Respiration and Circulation
Control and Co-ordination
- Control and Co-ordination
- Nervous System in Hydra
- Nervous System in Planaria (Flatworm)
- Neural Tissue
- Neuron (Or Nerve Cell)
- Neuroglial Cells (Or Glial Cells)
- Human Nervous System
- Central Nervous System (CNS)
- The Human Brain
- The Spinal Cord
- Peripheral Nervous System > Somatic Nervous System
- Sensory Receptors
- The Human Eye
- Human Ear
- Disorders of Nervous System
- Chemical Coordination
- Human Endocrine System
- The Hypothalamus
- Pituitary Gland or Hypophysis Gland
- The Pineal Gland
- Thyroid Gland
- Parathyroid Gland
- Thymus Gland
- Adrenal Gland (Suprarenal Gland)
- Pancreas (Islets of Langerhans)
- Reproductive Glands (Gonads)
- Synapse
- Types of Synapse
- Transmission of Nerve Impulse
- Generation of nerve impulse
- Reflex Action
- Peripheral Nervous System > Autonomic Nervous System
- Diffuse Endocrine Glands
- Overview of Control and Co-ordination
Human Health and Diseases
- Defence System in Our Body: Immune System
- Structure of Antibody
- Disease
- Protozoan Diseases
- Helminthic Diseases
- Bacterial Diseases
- Viral Diseases
- Fungal Diseases
- Vector Borne Diseases
- Cancer
- Drug Abuse
- Concept of Adolescence
- Addiction
- Vaccines and Vaccination
- Blood Transfusion and Blood Groups (ABO and Rh system)
Human Reproduction
Enhancement of Food Production
- Improvement in Food Production
- Plant Breeding
- Tissue Culture
- Single Cell Protein (SCP)
- Biofortification
- Animal Husbandry (Livestock)
- Animal Husbandry (Livestock) > Animal Breeding
- Dairy (Livestock) Farm Management
- Poultry Farm Management
- Animal Husbandry (Livestock) > Apiculture (Bee Farming)
- Animal Husbandry (Livestock) > Pisciculture (Fish Farming)
- Sericulture
- Lac Culture
- Microbes in Human Welfare
- Microbes in Industrial Products
- Microbes in Sewage Treatment
- Microbes in Energy Generation
- Microbes as Biocontrol Agents
- Microbes as Biofertilizers
- Microbial Role in Dairy Products
- Overview of Enhancement of Food Production
Biotechnology
- Biotechnology
- Principles of Biotechnology
- Methodology for rDNA Technology
- Crop Biotechnology > Hybrid Seeds
- Bioethics
- Effects of Biotechnology on the Environment
- Biopiracy
- Transgenic Plants
- Effects of Biotechnology on Human Health
- Tools and techniques for gene cloning/ rDNA technology
- Crop Biotechnology > Genetically Modified Organisms (GMOs)
- Overview of Biotechnology
Organisms and Populations
- Organisms and the Environment Around
- Habitat
- Niche
- Adaptations and Its Types
- Population
- Population Interactions
- Organisms and Populations
- Population Growth
- Positive Interactions > Mutualism (Symbiosis)
- Negative Interactions > Competition
- Negative Interactions > Parasitism
- Negative Interactions > Predation
- Positive Interactions > Commensalism
- Overview of Organisms and Populations
Ecosystems and Energy Flow
Biodiversity, Conservation and Environmental Issues
- Biodiversity
- Levels of Biodiversity
- Patterns of Biodiversity
- Biodiversity Current Scenario
- Loss of Biodiversity
- Conservation of Wildlife
- Biological Diversity Act, 2002
- Environmental Issues
- Noise Pollution
- Green House Effect
- Preventive Measures of Green House Effect
- Deforestation and Its Causes
- Global Warming
- Preventive Measures of Global Warming
- Mission Harit Maharashtra
- Conservation of Biodiversity
- Overview of Biodiversity, Conservation and Environmental Issues

Maharashtra State Board: Class 12

Definition: Polymerase Chain Reaction (PCR)

The in-vitro technique used to amplify a specific segment of DNA, producing millions of copies from a small amount of DNA in a short time using repeated thermal cycles, is called polymerase chain reaction (PCR).

Maharashtra State Board: Class 12

Key Points: Mechanism of PCR

Step	Temperature	Process	Result
Denaturation	90–98 °C	Double-stranded DNA separates into single strands	Template strands formed
Annealing	40–60 °C	Primers bind to complementary sequences on DNA	Primer-template complex formed
Extension (Elongation)	70–75 °C	Taq DNA polymerase adds nucleotides to primers	New DNA strands synthesized
Completion of One Cycle	—	One complete set of three steps	DNA quantity doubles
Repeated Cycles	—	Steps repeated 20–30 times	Millions of DNA copies formed

Maharashtra State Board: Class 12

Key Points: Methodology for rDNA Technology

Stage	Description	Key Tools / Examples
Isolation of DNA (Gene)	Desired gene is isolated, purified, and cleaved using restriction enzymes	Restriction endonucleases
Insertion into Vector	Foreign (passenger) DNA is inserted into plasmid/phage vector to form rDNA	DNA ligase, pBR322, λ phage
Transfer into Host	Recombinant DNA is introduced into competent host cell	Transformation, Ca²⁺, E. coli, Agrobacterium
Selection of Transformants	Recombinant cells are identified using marker genes	Antibiotic resistance (Ampᴿ, Tetᴿ)
Multiplication	Selected transformed cells are cultured and multiplied	Culture media
Expression of Gene	Desired product is synthesized and purified	Bioreactors, downstream processing