मराठी

Artificial Chromosomes as Vectors

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CISCE: Class 12

Key Points: Artificial Chromosomes as Vectors

Feature Bacterial Artificial Chromosomes (BACs) Yeast Artificial Chromosomes (YACs)
Source Derived from bacterial F-plasmid Derived from yeast (Saccharomyces cerevisiae) DNA
DNA insert size 100–300 kb (up to 350 kb) Up to 1 million base pairs
Stability More stable Less stable than BACs
Host cell E. coli Yeast cells
Important elements oriS, repE, parA, parB, antibiotic resistance, T7 & SP6 promoters Telomere, centromere, ori, selectable markers
Main use Genome mapping and sequencing Cloning very large eukaryotic genes
Special feature Maintains low copy number Behaves like a true yeast chromosome
Application Used in Human Genome Project (HGP) Used in Human Genome Project (HGP)
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