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Applications of Tissue Cultures in Crop Improvement

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CISCE: Class 12

Key Points: Applications of tissue cultures in crop improvement

  • Micropropagation – Rapid clonal multiplication of genetically identical plants (somaclones) in a short time; widely used for banana, potato, coconut and ornamentals.
  • Production of disease-free plants – Virus-free plants are produced through shoot apical meristem culture, especially in banana, potato, sugarcane and citrus.
  • Production of haploids – Anther and pollen culture produce haploid plants, helping in quick development of pure homozygous lines and reducing breeding time.
  • Embryo rescue – Immature or aborting embryos from difficult crosses are cultured to obtain viable hybrid plants, especially in fruit crops.
  • Induction and selection of mutants – Mutations are induced in cell cultures using chemicals, and useful resistant mutants are selected and regenerated.
  • Somaclonal variation – Genetic variability produced during tissue culture is used to develop disease-resistant, early-maturing and high-yielding varieties.
  • Protoplast technology and somatic hybridization – Fusion of protoplasts from different species enables transfer of useful genes beyond sexual compatibility barriers.
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