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Features of an Ideal Vector

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CISCE: Class 12

Key Points: Features of an Ideal Vector

  • Origin of replication (ori): Enables the vector and inserted DNA to replicate inside the host cell and controls copy number.
  • Selectable marker: Marker genes (e.g., antibiotic resistance) help identify and select transformed and recombinant cells.
  • Cloning sites: Presence of unique restriction enzyme sites allows insertion of foreign DNA without fragmenting the vector.
  • Small size: Small vectors are easy to isolate, handle, and manipulate during cloning.
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