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Plasmids - pUC Vectors

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CISCE: Class 12

Key Points: pUC Vectors

Feature Description
Name pUC series plasmid vectors
Size About 2,700 base pairs
Selectable marker Ampicillin resistance gene (ampR)
Origin of replication Derived from pBR322
Reporter gene lac Z gene from E. coli
Cloning principle Insertional inactivation of lac Z gene
Vector pairs pUC8 & pUC9, pUC18 & pUC19, pUC118 & pUC119
Special feature Paired vectors have reversed orientation of restriction sites
Advantage Allows isolation and study of both DNA strands
Uses Gene cloning, sequencing, mutagenesis
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