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Tools of Recombinant DNA Technology

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CISCE: Class 12

Key Points: Tools of Recombinant DNA Technology

  • Recombinant DNA technology depends on the isolation, cutting, and joining of DNA fragments to form chimeric DNA.
  • Restriction enzymes act as molecular scissors that cut DNA at specific sequences to obtain desired genes.
  • DNA ligase joins DNA fragments by forming covalent bonds, producing recombinant DNA molecules.
  • Cloning vectors are DNA molecules that carry foreign DNA into host cells for replication.
  • Common vectors include plasmids, bacteriophages, cosmids, and phasmids, which replicate independently inside host cells.
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