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Applications of Biotechnology in Health and Medicine - Genetically Engineered Insulin

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CISCE: Class 12

Key Points: Genetically Engineered Insulin

  • Earlier insulin was obtained from animals, which caused allergic reactions and could not meet large-scale demand.
  • Recombinant DNA technology enabled large-scale production of human insulin, first produced in 1983 as Humulin®.
  • Human insulin consists of two polypeptide chains (A and B) that are produced separately in E. coli using plasmid vectors.
  • The purified A and B chains are joined by disulphide bonds to form active human insulin.
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