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Formation of First Recombinant DNA

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Estimated time: 2 minutes
CBSE: Class 12

Key Points: Formation of First Recombinant DNA

  • The first recombinant DNA was created by Stanley Cohen and Herbert Boyer (1972) by linking the antibiotic resistance gene with a plasmid of Salmonella typhimurium.
  • Restriction enzymes (molecular scissors) cut DNA at specific locations; ligase joins the cut ends to link the gene with the plasmid.
  • Plasmids act as vectors to transfer the attached DNA into the host organism.
  • Recombinant DNA transferred into E. coli replicated using the host's DNA polymerase — making multiple copies, called cloning.
  • Steps in genetic modification - identify desirable genes → introduce into host → maintain and transfer DNA to progeny.
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