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DNA Replication - The Machinery and the Enzymes

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Estimated time: 2 minutes
CBSE: Class 12

Key Points: The Machinery and the Enzymes

  • Main enzyme = DNA-dependent DNA polymerase; polymerises at ~2000 bp/sec; mistakes cause mutations.
  • Deoxyribonucleoside triphosphates act as substrates and provide energy for polymerisation.
  • Replication occurs at the replication fork; only in the 5' to 3' direction; one strand = continuous, the other = discontinuous; fragments are joined by DNA ligase.
  • Replication starts at a fixed point called the origin of replication; DNA polymerase cannot initiate randomly.
  • In eukaryotes, replication occurs in the S-phase; failure of cell division after replication causes polyploidy.
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