हिंदी

Tools of Recombinant DNA Technology - Cloning Vectors

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Estimated time: 5 minutes
CBSE: Class 12

Key Points: Cloning Vectors

Feature Origin of Replication (ori) Selectable Marker Cloning Sites Vectors for Plants/Animals
Meaning DNA replication starting point Identifies and eliminates non-transformants Recognition sites for restriction enzymes Techniques from bacteria/viruses to transfer genes
Function Allows DNA to replicate in host cells Permits growth of transformants Simplifies linking of foreign DNA Delivers DNA to eukaryotic cells
Copy Number Control Controls the copy number of linked DNA
Examples High copy number vectors Antibiotic resistance genes (ampicillin, tetracycline) Specific restriction sites in genes Agrobacterium tumefaciens Ti plasmid, retroviruses
Methodology Used to obtain high copies of target DNA Antibiotics select transformed cells Foreign DNA inserted at restriction sites Modified pathogens used for gene delivery
Application DNA amplification in the host Selection of transformants Gene cloning process Gene transfer into plants and animals
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