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The Hershey-Chase Experiment

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Estimated time: 2 minutes
Maharashtra State Board: Class 12

Key Points: The Hershey-Chase Experiment

  • In 1952, Alfred Hershey and Martha Chase proved that DNA is the genetic material using bacteriophages and E. coli bacteria.
  • They used radioactive isotopes: ³²P to label DNA and ³⁵S to label proteins.
  • Viruses grown in ³²P medium had radioactive DNA, while those grown in ³⁵S medium had radioactive protein.
  • These labelled viruses were allowed to infect E. coli, and then blending and centrifugation were done to separate viral coats.
  • Only bacteria infected with ³²P-labelled viruses became radioactive, showing that DNA entered the bacterial cells.
  • Bacteria infected with ³⁵S-labelled viruses were not radioactive, proving proteins did not enter the cells; hence, DNA is the genetic material.
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