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प्रश्न
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
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उत्तर
In a gene, cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector (the step would not be affected) and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant/plasmid DNA, in the absence of a selectable marker, it will be difficult to distinguish between transformants and non-transformants, because role of the selectable marker is in the selection of transformants.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Describe the role of CaCl2 in the preparation of competent cells?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
Which of the following is a group of vectors used for gene transfer into animals?
