Advertisements
Advertisements
Question
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Advertisements
Solution
In a gene, cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector (the step would not be affected) and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant/plasmid DNA, in the absence of a selectable marker, it will be difficult to distinguish between transformants and non-transformants, because role of the selectable marker is in the selection of transformants.
APPEARS IN
RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
A clone is a ______.
Which of the following is correctly matched?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the primary function of a vector in recombinant DNA technology?
What is the basic biological nature of plasmids when used as cloning vectors?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
