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Question
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
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Solution
Importance of Selectable Marker:-
(i) It helps in identifying and eliminating non-transformants.
(ii) It selectively permitting the growth of the transformant.
e.g- The genes encoding resistance to antibiotics such as Ampicillin, Chloramphenicol Tetracyclin or Kanamycin are considered useful selectable markers for E.coli because normally E-coli does not carry resistance against any of these antibiotics.
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RELATED QUESTIONS
Write the role of ‘restriction sites’ in the cloning vector pBR322.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following statement is not true for a clone?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What does ‘competent’ refer to in competent cells used in transformation experiments?
Name the regions marked A, B and C.
What is the primary function of a vector in recombinant DNA technology?
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
