Advertisements
Advertisements
प्रश्न
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Advertisements
उत्तर
Importance of Selectable Marker:-
(i) It helps in identifying and eliminating non-transformants.
(ii) It selectively permitting the growth of the transformant.
e.g- The genes encoding resistance to antibiotics such as Ampicillin, Chloramphenicol Tetracyclin or Kanamycin are considered useful selectable markers for E.coli because normally E-coli does not carry resistance against any of these antibiotics.
APPEARS IN
संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Write the role of ‘restriction sites’ in the cloning vector pBR322.
A clone is a ______.
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
