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प्रश्न
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
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उत्तर
Importance of Selectable Marker:-
(i) It helps in identifying and eliminating non-transformants.
(ii) It selectively permitting the growth of the transformant.
e.g- The genes encoding resistance to antibiotics such as Ampicillin, Chloramphenicol Tetracyclin or Kanamycin are considered useful selectable markers for E.coli because normally E-coli does not carry resistance against any of these antibiotics.
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संबंधित प्रश्न
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
What is ‘Ori’ ? State its importance during cloning of a vector.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
