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प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
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उत्तर
Origin of Replication (Ori):- This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
Importance of Ori:-
(i) It is responsible for controlling the copy number & the linked DNA.
(ii) In cloning if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.
APPEARS IN
संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
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Name the regions marked A, B and C.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
Which of the following is not a cloning vector?
