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प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
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उत्तर
Origin of Replication (Ori):- This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
Importance of Ori:-
(i) It is responsible for controlling the copy number & the linked DNA.
(ii) In cloning if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.
APPEARS IN
संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Which of the following statement is not true for a clone?
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What does ‘competent’ refer to in competent cells used in transformation experiments?
Describe the role of CaCl2 in the preparation of competent cells?
What is the primary role of a cloning site in a vector?
