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प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
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उत्तर
Origin of Replication (Ori):- This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
Importance of Ori:-
(i) It is responsible for controlling the copy number & the linked DNA.
(ii) In cloning if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.
APPEARS IN
संबंधित प्रश्न
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
A suitable vector for gene cloning in higher organism is ______.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
Which of the following is not a cloning vector?
Which of the following describes the function of a selectable marker in a cloning vector?
