Advertisements
Advertisements
प्रश्न
Describe briefly the following:
Origin of replication
Advertisements
उत्तर
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
APPEARS IN
संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Name the regions marked A, B and C.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
