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प्रश्न
Describe briefly the following:
Origin of replication
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उत्तर
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
Which of the following is not a cloning vector?
