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प्रश्न
Describe briefly the following:
Origin of replication
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उत्तर
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
