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प्रश्न
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
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उत्तर
'YAC' → Yeast Artificial Chromosome :
It is used as a cloning vector for cloning DNA fragments in the suitable host so that DNA sequencing can be done.
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संबंधित प्रश्न
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
The genetic material of virus includes:
A clone is a ______.
A suitable vector for gene cloning in higher organism is ______.
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
