Question

The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:

पर्याय

• The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.

• Gene ‘X’ is responsible for recognition sites and ‘Y’ is responsible for antibiotic resistance.

• The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.

• The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.

Answer 1

The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.

Explanation:

In the above diagram, ‘X’ stands for ori, which regulates the number of copies of the linked DNA, and ‘Y’ stands for rop, which codes for a protein involved in plasmid replication.