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प्रश्न
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
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उत्तर
'YAC' → Yeast Artificial Chromosome :
It is used as a cloning vector for cloning DNA fragments in the suitable host so that DNA sequencing can be done.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Which of the following statement is not true for a clone?
Which of the following is not a cloning vector?
Describe the role of CaCl2 in the preparation of competent cells?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
