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प्रश्न
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
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उत्तर
'YAC' → Yeast Artificial Chromosome :
It is used as a cloning vector for cloning DNA fragments in the suitable host so that DNA sequencing can be done.
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संबंधित प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
Name the regions marked A, B and C.

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:

