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प्रश्न
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
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उत्तर
T-DNA is the only essential part required to make Ti plasmid a cloning vector. The plasmid is disarmed by deleting the tumour-inducing genes in the plasmid so that it becomes an effective cloning vector and remove it harmful effect.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Describe briefly the following:
Origin of replication
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
A clone is a ______.
Which of the following is correctly matched?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Describe the role of CaCl2 in the preparation of competent cells?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
Which of the following describes the function of a selectable marker in a cloning vector?
