Advertisements
Advertisements
प्रश्न
What is meant by gene cloning?
Advertisements
उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
APPEARS IN
संबंधित प्रश्न
The bacterium which causes a plant disease called crown gall is _______________.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is the role of Taq-polymerase in PCR technology?
Bt-cotton shows adverse effect on the population of which butterfly?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
____________ cuts the DNA within the specific positions.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Polymerase Chain Reaction is made possible by ____________.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The gene transfer between unrelated plants is brought about by ____________.
The source of taq Polymerase used in PCR is a ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Which amino acid is coded by the initiation codon?
In vitro amplification of DNA segments is called______.
Expand YAC used in the field of Biotechnology.
Which one of the following enzymes is used to join DNA fragments?
