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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Distinguish between RNA and DNA.
Name the most commonly used bioreactor and describe its working.
Expand the following abbreviation: BAC
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Define vector? write any two examples,
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Name and describe the technique that helps in separation of DNA fragments.
Which molecules are called molecular scissors? Why are they called so?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Name the plant disease caused by Agrobacterium tumefaciens.
The PCR technique is used for ______.
Name two restriction enzymes used in PCR.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Name the enzymes that are called molecular scissors.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
Which of the following best describes the relationship between genetic engineering and recombinant DNA?
