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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
How has the development of bioreactor helped in biotechnology?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
The biological scissor is ______.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
The enzyme restriction endonuclease ______.
Which of the following is an INCORRECT match?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Lytic cycle involves bacteriophage and ____________.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Name two restriction enzymes used in PCR.
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
What are the different types of electrophoresis?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
The restriction enzymes used as molecular scissors are type of ______.
The three basic steps in genetic modification are:
The origin of replication is best described as:
