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Question
What is meant by gene cloning?
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Solution
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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RELATED QUESTIONS
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
What are plasmids?
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Describe two vectors less methods of gene transfer used in rDNA technology.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is PCR? Explain different steps involved in it.
From the following identify the methods of electrophoresis.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
____________ cuts the DNA within the specific positions.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The gene transfer between unrelated plants is brought about by ____________.
Which molecules are called molecular scissors? Why are they called so?
The source of taq Polymerase used in PCR is a ______.
Nucleic acid segment tagged with a radioactive molecule is called ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
______technique is used for in vitro gene cloning or gene multiplication.
In vitro amplification of DNA segments is called______.
How many sets of primers are required in each cycle of PCR?
Which one of the following enzymes is used to join DNA fragments?
