Advertisements
Advertisements
Question
What is meant by gene cloning?
Advertisements
Solution
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
APPEARS IN
RELATED QUESTIONS
State the role of DNA ligase in biotechnology.
Distinguish between RNA and DNA.
How has the development of bioreactor helped in biotechnology?
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
____________ cuts the DNA within the specific positions.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Human insulin is produced by culturing the genetically engineered ______
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
The source of taq Polymerase used in PCR is a ______.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The polymerase chain reaction is a powerful technique to ______.
What are the different types of electrophoresis?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
