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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Name the most commonly used bioreactor and describe its working.
What are the restriction endonucleases? Give the rules of their nomenclature.
What is the cell that receives a recombinant gene called?
The technique which involves addition or deletion of genes is ______.
What is PCR? Explain different steps involved in it.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
What is true for plasmid?
Which of these are most widely used in genetic engineering?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
What is polymerase chain reaction (PCR)?
Explain the mechanism of PCR.
Name the source of thermostable DNA polymerase.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
