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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Give an example of the source of thermostable enzyme DNA polymerase.
The biological scissor is ______.
What are the restriction endonucleases? Give the rules of their nomenclature.
Why are bio-fertilizers preferred over chemical fertilizers?
The bacterium which causes a plant disease called crown gall is _______________.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
ECoRI is obtained from ______.
Alu-I is obtained from which organism?
Define vector? write any two examples,
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the characterization techniques that are based on molecular weights of compounds.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which of the following viruses has the capacity to produce lysozymes?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
