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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
The technique which involves addition or deletion of genes is ______.
What is plasmid?
Define vector? write any two examples,
What is gene cloning? Explain different tools used for it.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Polymerase Chain Reaction is made possible by ____________.
Lytic cycle involves bacteriophage and ____________.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Which molecules are called molecular scissors? Why are they called so?
Which of the following viruses has the capacity to produce lysozymes?
Which vector is mostly used in rDNA technology in plants?
Write steps involved in gene amplification.
Name the source of thermostable DNA polymerase.
The restriction enzymes used as molecular scissors are type of ______.
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
