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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
The bacterium which causes a plant disease called crown gall is _______________.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
What is Palindromic sequence?
Alu-I is obtained from which organism?
Bt-cotton shows adverse effect on the population of which butterfly?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Identify the characterization techniques that are based on molecular weights of compounds.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Polymerase Chain Reaction is made possible by ____________.
Lytic cycle involves bacteriophage and ____________.
Human insulin is produced by culturing the genetically engineered ______
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Name the plant disease caused by Agrobacterium tumefaciens.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Describe three steps involved in mechanism of PCR.
What is the primary focus of genetic engineering?
