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प्रश्न
What is meant by gene cloning?
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उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Give the applications of the PCR technique.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is plasmid?
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
From the following identify the methods of electrophoresis.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Lytic cycle involves bacteriophage and ____________.
Human insulin is produced by culturing the genetically engineered ______
Annealing step of PCR operates at ______ °C.
Name the plant disease caused by Agrobacterium tumefaciens.
Restriction enzymes were discovered by ______.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
To be a cloning vector, a plasmid require ______.
Plasmid is ______.
The DNA polymerase enzyme used in PCR is obtained from______.
Which amino acid is coded by the initiation codon?
How many sets of primers are required in each cycle of PCR?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
