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प्रश्न
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
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उत्तर
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is gene cloning? Explain different tools used for it.
Which of the following is TRUE regarding EcoR I?
The time taken to complete one cycle of PCR is around ______.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
What is true for plasmid?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Describe three steps involved in mechanism of PCR.
Plasmid is ______.
______technique is used for in vitro gene cloning or gene multiplication.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Give a reason for the following:
DNA cannot enter directly into the host cell.
