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प्रश्न
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
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उत्तर
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
What are the restriction endonucleases? Give the rules of their nomenclature.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
What is plasmid?
Identify the characterization techniques that are based on molecular weights of compounds.
From the following identify the methods of electrophoresis.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Exonuclease performs the function of ____________.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Name a recombinant vaccine that is currently being used in vaccination programme.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Restriction enzymes were discovered by ______.
Which of these are most widely used in genetic engineering?
What is polymerase chain reaction (PCR)?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
