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प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
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उत्तर
The correct sequence of the steps involved in biotechnological reaction are
Genomic DNA template
↓
Denaturation of ds-DNA
↓
Nucleotides provided
↓
Primers
↓
Thermostable DNA polymerase (from Thermus aquaticus )
↓
Enzyme DNA polymerase
↓
Chemically synthesised oligonucleotides
↓
Complementary region of DNA
↓
In vitro synthesis of copies of DNA of interest
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संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Polymerase chain reaction is most useful in ______.
What is the role of Taq-polymerase in PCR technology?
Which of the following DNA sequence is recognised and cut by EcoR I?
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which of the following is not required in the preparation of a recombinant DNA molecule?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
What is polymerase chain reaction (PCR)?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
