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प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
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उत्तर
The correct sequence of the steps involved in biotechnological reaction are
Genomic DNA template
↓
Denaturation of ds-DNA
↓
Nucleotides provided
↓
Primers
↓
Thermostable DNA polymerase (from Thermus aquaticus )
↓
Enzyme DNA polymerase
↓
Chemically synthesised oligonucleotides
↓
Complementary region of DNA
↓
In vitro synthesis of copies of DNA of interest
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संबंधित प्रश्न
Explain types of enzymes used in biotechnology?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Exonuclease performs the function of ____________.
Nucleic acid segment tagged with a radioactive molecule is called ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
What is meant by gene cloning?
Which of these are most widely used in genetic engineering?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
What is the function of DNA ligase in the creation of recombinant DNA?
