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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
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ii. Ownership of technology and its sociopolitical impact.
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| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
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