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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
Expand the following abbreviation: BAC
The bacterium which causes a plant disease called crown gall is _______________.
Ti plasmid being used for introducing genes in plants obtained from ______.
Define vector? write any two examples,
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
____________ cuts the DNA within the specific positions.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The vector Baculovirus is used for gene transfer in ____________.
The gene transfer between unrelated plants is brought about by ____________.
EcoRl & Hind III are the examples of ______
What is true for plasmid?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
The PCR technique is used for ______.
The polymerase chain reaction is a powerful technique to ______.
Write a short note on vectors.
Which amino acid is coded by the initiation codon?
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
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| Vector A | Vector B | Vector C |
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
