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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
What are plasmids?
Give the applications of the PCR technique.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is Palindromic sequence?
What is PCR? Explain different steps involved in it.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the CORRECT sequence of steps in PCR.
The time taken to complete one cycle of PCR is around ______.
Identify the characterization techniques that are based on molecular weights of compounds.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
______ is a Type I restriction enzyme.
Polymerase Chain Reaction is made possible by ____________.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Name two restriction enzymes used in PCR.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Importance of PCR.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
