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प्रश्न
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
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उत्तर
i. If a PCR machine fails to lower the temperature below 70°C then the annealing step (in which primers attach to the respective ends of the DNA template) would be affected.
ii. The annealing step of PCR requires a temperature ranging between 40°C to 60°C therefore if a PCR machine fails to lower the temperature below 70°C, primers will not attach to the DNA templates.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
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(e) Genomic DNA template
(f) Nucleotides provided
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(h) Thermostable DNA-polymerase (from Thermus aquaticus)
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