Advertisements
Advertisements
Question
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Advertisements
Solution
i. If a PCR machine fails to lower the temperature below 70°C then the annealing step (in which primers attach to the respective ends of the DNA template) would be affected.
ii. The annealing step of PCR requires a temperature ranging between 40°C to 60°C therefore if a PCR machine fails to lower the temperature below 70°C, primers will not attach to the DNA templates.
APPEARS IN
RELATED QUESTIONS
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Why are bio-fertilizers preferred over chemical fertilizers?
Enlist and write in brief about the different biological tools required in rDNA technology.
The technique which involves addition or deletion of genes is ______.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
The source of taq Polymerase used in PCR is a ______.
Name the source of thermostable DNA polymerase.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
