Advertisements
Advertisements
Question
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Advertisements
Solution
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
APPEARS IN
RELATED QUESTIONS
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
What is plasmid?
Alu-I is obtained from which organism?
Define vector? write any two examples,
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the CORRECT sequence of steps in PCR.
Which of the following device is required for PCR?
Exonuclease performs the function of ____________.
EcoRl & Hind III are the examples of ______
A hind of biotechnology involving manipulation of DNA is ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
The PCR technique is used for ______.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Write a short note on vectors.
