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Question
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
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Solution
(i) EcoRI
(ii)
| 5'CAG3' | 5'AATTCTTA3' |
| 3'GTCTTAA 5' | 3'GAAT5' |
(iii) Because they create hydrogen connections with their complementary cut sections, these are known as sticky ends.
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