Advertisements
Advertisements
प्रश्न
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Advertisements
उत्तर
(i) EcoRI
(ii)
| 5'CAG3' | 5'AATTCTTA3' |
| 3'GTCTTAA 5' | 3'GAAT5' |
(iii) Because they create hydrogen connections with their complementary cut sections, these are known as sticky ends.
APPEARS IN
संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
EcoRl & Hind III are the examples of ______
What is meant by gene cloning?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
The PCR technique is used for ______.
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The polymerase chain reaction is a powerful technique to ______.
Give a reason for the following:
DNA cannot enter directly into the host cell.
