Advertisements
Advertisements
प्रश्न
What would be the molar concentration of human DNA in a human cell?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
Advertisements
उत्तर
Human diploid cell contains about 6 × 10–12 g DNA.
Human genome size ≈ 6.4 × 109 base pairs
Average mass of 1 base pair ≈ 660 g mol–1
Molar mass of DNA = 6.4 × 109 × 660
= 4.2 × 1012 g mol–1
Moles of DNA per cell = `(6xx10^-12)/(4.2xx10^12)`
= 1.4 × 10–24 mol
Taking average cell volume = 10–12 L
Concentration = `(1.4xx10^-24)/10^-12`
= 1.4 × 10–12 M
APPEARS IN
संबंधित प्रश्न
What are plasmids?
Give one significant difference between:
Electroporation and Gene Gun.
What is the cell that receives a recombinant gene called?
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Explain types of enzymes used in biotechnology?
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
______ is a Type I restriction enzyme.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Which molecules are called molecular scissors? Why are they called so?
Which one is a true statement regarding DNA polymerase used in PCR?
A hind of biotechnology involving manipulation of DNA is ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The polymerase chain reaction is a powerful technique to ______.
Write steps involved in gene amplification.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
