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प्रश्न
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
विकल्प
S' _ _ _ A _ G ↓ _ C _ T _ _ _ 3'
3' _ _ _ T _ C _ ↑ _ G _ A _ _ _ S'
5' _ _ _ G _ _ G _ A _ T _ C _ C _ _ _ 3'
3' _ _ _ C _ C _ T _ A _ G _ _ G _ _ _ 5'
5' _ _ G _ T _ C _ ↓ _ G _ A _ C _ _ 3'
3' _ _ C _ A _ G _ ↑ _ C _ T _ G _ _ 5'
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
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उत्तर
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
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संबंधित प्रश्न
The technique which involves addition or deletion of genes is ______.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
EcoRl & Hind III are the examples of ______
Name and describe the technique that helps in separation of DNA fragments.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
