Advertisements
Advertisements
Question
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Options
S' _ _ _ A _ G ↓ _ C _ T _ _ _ 3'
3' _ _ _ T _ C _ ↑ _ G _ A _ _ _ S'
5' _ _ _ G _ _ G _ A _ T _ C _ C _ _ _ 3'
3' _ _ _ C _ C _ T _ A _ G _ _ G _ _ _ 5'
5' _ _ G _ T _ C _ ↓ _ G _ A _ C _ _ 3'
3' _ _ C _ A _ G _ ↑ _ C _ T _ G _ _ 5'
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
Advertisements
Solution
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
APPEARS IN
RELATED QUESTIONS
What are plasmids?
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
What is plasmid?
From the following identify the methods of electrophoresis.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The gene transfer between unrelated plants is brought about by ____________.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Explain the mechanism of PCR.
