Advertisements
Advertisements
Question
Write a short note on vectors.
Short/Brief Note
Advertisements
Solution
- Vectors are DNA molecules that transport and reproduce foreign DNA segments within the host cell.
- Vectors include plasmids, bacteriophages (M13, lambda virus), cosmids, phagemids, BACs, YACs (yeast artificial chromosomes), transposons, baculoviruses, and mammalian artificial chromosomes (MACs).
- Plasmid vectors (pBR 322, pUC, Ti plasmid) and bacteriophages (lambda phage, M13 phage) are the most often utilised vectors.
shaalaa.com
Is there an error in this question or solution?
APPEARS IN
RELATED QUESTIONS
Give one significant difference between:
Electroporation and Gene Gun.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
The enzyme restriction endonuclease ______.
What is the role of Taq-polymerase in PCR technology?
What is PCR? Explain different steps involved in it.
From the following identify the methods of electrophoresis.
Annealing step of PCR operates at ______ °C.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
In vitro amplification of DNA segments is called______.
