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Question
Write a short note on vectors.
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Solution
- Vectors are DNA molecules that transport and reproduce foreign DNA segments within the host cell.
- Vectors include plasmids, bacteriophages (M13, lambda virus), cosmids, phagemids, BACs, YACs (yeast artificial chromosomes), transposons, baculoviruses, and mammalian artificial chromosomes (MACs).
- Plasmid vectors (pBR 322, pUC, Ti plasmid) and bacteriophages (lambda phage, M13 phage) are the most often utilised vectors.
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OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
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Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
