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प्रश्न
Write a short note on vectors.
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उत्तर
- Vectors are DNA molecules that transport and reproduce foreign DNA segments within the host cell.
- Vectors include plasmids, bacteriophages (M13, lambda virus), cosmids, phagemids, BACs, YACs (yeast artificial chromosomes), transposons, baculoviruses, and mammalian artificial chromosomes (MACs).
- Plasmid vectors (pBR 322, pUC, Ti plasmid) and bacteriophages (lambda phage, M13 phage) are the most often utilised vectors.
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संबंधित प्रश्न
Expand the following abbreviation: BAC
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Enlist and write in brief about the different biological tools required in rDNA technology.
ECoRI is obtained from ______.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The PCR technique is used for ______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Expand YAC used in the field of Biotechnology.
