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प्रश्न
How many sets of primers are required in each cycle of PCR?
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उत्तर
Two sets of primers are required in each cycle of PCR.
संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
How does EcoRI differ from an exonuclease?
