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प्रश्न
How many sets of primers are required in each cycle of PCR?
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उत्तर
Two sets of primers are required in each cycle of PCR.
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
What would be the molar concentration of human DNA in a human cell?
What are the restriction endonucleases? Give the rules of their nomenclature.
The gene transfer between unrelated plants is brought about by ____________.
Exonuclease performs the function of ____________.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Expand the following acronym:
BAC
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
The three basic steps in genetic modification are:
