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प्रश्न
The enzyme restriction endonuclease ______.
विकल्प
cuts double strand of DNA
joins strand of DNA
cuts RNA strand
cuts single stranded DNA
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उत्तर
The enzyme restriction endonuclease cuts double strand of DNA.
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संबंधित प्रश्न
The biological scissor is ______.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
The bacterium which causes a plant disease called crown gall is _______________.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Identify the characterization techniques that are based on molecular weights of compounds.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
What is true for plasmid?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
