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प्रश्न
In vitro amplification of DNA or RNA segment is known as ___________.
विकल्प
chromatography
Southern blotting
polymerase chain reaction
gel electrophoresis
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उत्तर
In vitro amplification of DNA or RNA segment is known as the polymerase chain reaction.
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संबंधित प्रश्न
The enzyme restriction endonuclease ______.
Polymerase Chain Reaction is made possible by ____________.
Which of the following statements is correct with respect to cellular respiration?
DNA fingerprinting refers to ______.
Name the plant disease caused by Agrobacterium tumefaciens.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of these are most widely used in genetic engineering?
Describe three steps involved in mechanism of PCR.
Write a short note on vectors.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
