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प्रश्न
In vitro amplification of DNA or RNA segment is known as ___________.
विकल्प
chromatography
Southern blotting
polymerase chain reaction
gel electrophoresis
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उत्तर
In vitro amplification of DNA or RNA segment is known as the polymerase chain reaction.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Polymerase chain reaction is most useful in ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Write a short note on vectors.
How does EcoRI differ from an exonuclease?
The origin of replication is best described as:
