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प्रश्न
In vitro amplification of DNA or RNA segment is known as ___________.
विकल्प
chromatography
Southern blotting
polymerase chain reaction
gel electrophoresis
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उत्तर
In vitro amplification of DNA or RNA segment is known as the polymerase chain reaction.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
ECoRI is obtained from ______.
What is PCR? Explain different steps involved in it.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
The time taken to complete one cycle of PCR is around ______.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
What is EcoRI?
