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प्रश्न
ECoRI is obtained from ______.
विकल्प
Escherichia coli R13
Escherichia coli Ry13
Escherichia coli R225
Escherichia coli RC
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उत्तर
ECoRI is obtained from Escherichia coli Ry13.
APPEARS IN
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Distinguish between RNA and DNA.
What is gene cloning? Explain different tools used for it.
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Which of the following DNA sequence is recognised and cut by EcoR I?
The vector Baculovirus is used for gene transfer in ____________.
Which of these are most widely used in genetic engineering?
Plasmid is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
